Authors : Mohini Dhanulkar, Jayashree Tirpude, Nandita Pradhan, Anil Mohite, Harsha Khapekar, Prajakta Gahukar
Page Nos : 179-187
Description :
Histopathological examination of ovary tissue of African catfish C. gariepinus exposed to 250 mg/lit.2-MeTHF for long
term showed severe and variable effects. These includes degenerative change in ovary, vacuolization in the ooplasm,
damaged follicular lining and deshped oocytes. Intramuscular exposure of 10 mg 2-MeTHF showed vacuolization in
oocytes and degeneration of follicular wall in C. gariepinus. Exposure of 40 mg intramuscular dose of 2-MeTHF
showed histological abnormalities like development of intrafollicular space in oocytes, broken wall in vitellogenic
oocytes, decreased nucleoli in perinuclear stage, clumping of cytoplasm in mature oocytes in ovary of fish C.
gariepinus was observed in the present study. It was observed that number of mature oocytes have been reduced with
large follicular space due to the effect of 2-MeTHF. Similarly vacuoles in the vitelline follicules and broken albuginea
was also observed in the ovary. The generation of follicular atresia was observed in the present study due to the effect
of 2-MeTHF, which characterized by degeneration and necrosis such as dissolution and disappearance of the nucleus
and changes in the mitochondria and other organelles were found. When the fish was exposed to 500 mg/lit
concentration of 2-MeTHF and 20 mg intramuscular dose of 2-MeTHF cause degeneration of ovarian wall and
breakdown of vitellogenic epithelium and degeneration of granulose cell in secondary oocytes.
